Effects of mobile phone radiation on protein expression in different types of human primary endothelial cells (Human Brain EC)

Aim of the study

The project will determine whether human primary endothelial cells obtained from different blood vessels respond to mobile phone radiation in the same or different fashion.

Implementation

Human umbilical cord endothelial cells (HUVEC) and human brain capillary endothelial cells (HBMEC) are used as a model. The cell cultures are exposed to 1800MHz GSM radiation. Following the exposure, the proteins are extracted from the cells labelled with fluorescent dyes and separated using 2D-electrophoresis. The changes in the expression of proteins are detected with a scanner and analysed with a computer software. If the expression of certain proteins is altered by mobile phone radiation, these proteins will be extracted from the 2D-electrophoresis gels and identified using a mass spectrometry.

Dissemination and exploitation of results

The results will show whether it is possible that transcriptome and proteome may affect the cell response to the mobile phone radiation. The results will be published in 2009 in a peer-review journal and presented at an international conference.

Collaborators

Swiss Federal Institute of Technology, Zürich, Switzerland (Prof. Niels Kuster)

Timetable

2007 – 2008

Responsible scientist

Dariusz Leszczynski

Page updated 15/05/2009